Phage Display Libraries For Therapeutic Antibody Discovery

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Phage Display Libraries For Therapeutic Antibody Discovery

Phage display antibody library technology has played an important role in the remarkable progress of discovering and optimizing antibodies for diverse applications, specifically Monoclonal Antibody Drugs. The technology was firstly introduced by George smith and applied by john McCafferty. Here we compare nine phage display antibody libraries published in the last decades, which represent the state of the art in the discovery and development of therapeutic Antibody Drug Discovery using phage display. 

Here we will discuss the quality of the Phage display antibody library and the diverse kind of antibodies repertoires used as substrates to construct the libraries that are naive, synthetic, and semisynthetic. 

Overview – Phage Display Libraries

Antibody engineering techniques have been utilized to create chimeric or humanized antibodies by utilizing the murine regions or complementary determining regions aka CDRs.

Phage Display Libraries for Therapeutic Antibody Discovery

Antibodies phage display is versatile, in vitro selection Phage display antibody library technology that may be utilized to track down high-affinity antibodies specific to a wide variety of antigens. But specificity and high affinity are not the only attributes that result in successful therapeutic antibodies.

And other antibody quality attributes such as solubility, viscosity, expression yield, and thermal and long-term stability are vital to ensure the success of mAb candidates in biomanufacturing and clinical trials. All these biophysical properties of antibodies are strongly dependent on their amino acid sequence.

1. Naive Libraries

So a naive library does not create any assumptions about the diversity of the antibody repertoire. The rationale is that the human antibody repertoire evolves to recognize any aim with reasonable specificity and affinity.

Hence, the goal of developing libraries is to mirror the diversity of the human antibody repertoire while avoiding biases and redundancy due to the immunological history of the few individuals and rare polymorphic antibody genes present in the repertoire of the given ethnic group.

2. Synthetic Libraries

Synthetic repertoires are to increase the functionality of the antibody libraries by using well-expressed and developable scaffolds, targeting positions for diversification that do not disrupt the folding of the V regions, and choosing types and frequency of amino acids that facilitate the selection of diverse binder to any given goal.

3. Semisynthetic Libraries

Some iterations to improve the quality of synthetic have been performed with the design of HCDR3 being a constant theme and perhaps the main chance for improvement. The HCDR3 is an essential element in defining the specificity and affinity of antibodies, but it is also by far the most diverse region of the antigen-binding site and hence difficult to design. 

However, no method is available to reliably predict the HCDR3 structure, thus limiting our ability to properly design the diversity of this antigen binding-site region.

The review of the libraries with respect to the number of positive clones’ responses against the selected antibodies. Last we will highlight the current opportunities and challenges of phage display platforms and related display technologies.

Knowing Phage display antibody library GeNext Genomics participates in constructively producing monoclonal antibody drugs. Having expert scientists and trained professionals at work, GeNext Genomics focuses on creating high-quality STAT3 protein with a high amount of Protein Purification Techniques.