At GeNext Genomics, we are highly proud to serve the industry with the ability to cater for clients with antibody engineering services. These constitute each format, from the antibody fragments to the humanized antibodies.
scFV/Fab construction is the first and foremost step in antibody engineering. The scFV antibody fragments are of high advantage compared with the monoclonal antibodies (mAbs).With the Fab fragments being the critical component of the construction of antibody engineering services. These differ from scFVs and have one constant and variable domain with each heavy and light chain composition.
A chimeric antibody is regarded as one of the essential methods used in antibody engineering. The method combines the genetic material from one species source to another, say mouse and human being.
The function of the chimeric antibody is to retain the foreign antibodies antigen specificity and affinity, which makes it less rejected from the immune system. This promotes the comparison with ordinary mAbs.
This has the potential to decrease the immunogenicity of mAbs and maintain high specificity. In contrast to the chimeric antibody, the protein sequence of a humanized antibody is identical to the human variant. The non-human origins are present in the CDR (Complementarity Determining Region).
These humanized antibodies via antibody engineering services have anticancer targeted drug development and therapeutic applications.
Fc Fusion Proteins have an immunoglobulin Fc domain directly linked to another peptide of interest or a protein. These play a significant role in dimerization, in vivo half-life, receptor activation, and the affinity for cognate ligands. It expands the plasma half-life of the antibody and introduces mutation in the domain.
The antibody library technique for the antibody engineering services is of phage display. With the traditional hybridoma method, phage display is always ahead of advantage with therapeutic and diagnostic applications. It is regarded as novel antibodies, and we have such non-immune and immune libraries in bulk catering to clones and sets.
A recombinant expression can be created with a patent application, downstream development with the known protein sequence. Hybridoma sequencing, aka antibody sequencing, encodes the cDNA domains of the heavy and light cell line. With the antibody engineering services, you tend to have versatile sequencing of the species range.