Phage Display Antibody Library
Discover cutting-edge Phage Display Antibody Library Services with us. Leveraging the power of phage display technology, we provide tailored solutions for discovering high-affinity antibodies to meet your unique requirements. Our high-quality Antibody libraries, enable comprehensive exploration of potential CDR variants, ensuring thorough hit identification.
Our team, equipped with molecular biology expertise and dedicated to innovation, is committed to delivering what you need. Accelerate your monoclonal antibody development program with GeNext Genomics’ phage display services, and receive your antibodies within just 2 months!
Phage Display Technology
Phage display is a selection technique that involves genetic engineering of bacteriophages and repeated antigen-based selection and propagation. It offers a promising alternative for antibody generation outside the immune system, eliminating the need for laboratory animals. Antibody libraries are crafted from genomic information encoding antibody variable domains, sourced from B cells of either native or immune donors.
Diverse population gene pool across various disease background and healthy donors
DCloning of VH and VL in phagemid vector
Library preparation and transfection
Packaging and phage preparation
Biopanning against the target 4 round
ScFv generation and sequencing of binders
Final IgC cloned and expressed in HEK
Cancer/Infectious proteins injected into mice for immune response
Cloning of VH and VL and library generation
Biopanning against target/disease and functional assay
Identification of ScFv and its genetic sequence
Final igG cloned and expressed in HEK/CHO
Application and validation
Scale up of Antibodies
| Steps | Description | Deliverables |
|---|---|---|
| Library screening and biopanning | Selection and screening of the naïve or immune library. 4-5 rounds of biopanning to enrich binders | Progress report |
| ELISA screening | Screening and validation by ELISA to identify binders with desired specificity and affinity | Progress report |
| DNA extraction and sequencing | Extraction and sequencing of DNA from identified binders | Report DNA sequences |
| Development of full IgG | Gene synthesis Cloning of gene into a proprietary vector Transfection of host cell, screening, scale up & purification | Purified IgG antibody |
| Affinity Maturation | Enhancing the affinity and specificity of your antibodies through our affinity maturation services, optimizing their performance for various applications. | High affinity antibody |
| Antibody Characterization | Epitope mapping to determine the specific antigen binding site Binding kinetics analysis to measure the strength of antibody-antigen binding | Characterization report |
Expertise
Our team consists of experts with extensive experience in phage display technology and antibody discovery.
Quality
We prioritize quality at every step of the process to deliver reliable and high-performance antibody candidates.
Customization
Tailored solutions designed to address your specific project requirements.
Timely Delivery
Efficient project management ensures timely delivery of results to meet your deadlines.
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