Monoclonal Antibody Development

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Overview

From Antigen Preparation To Antibody Production, We Offer Comprehensive Support For All Stages Of Mouse Monoclonal Antibody Development, Including The Crucial Phases:

Animal Immunization

Fusion

Sub Cloning

Antibody Production

The End Results Consist Of The Purified Monoclonal Antibody And The Hybridoma Cell Line, While Our Skilled Project Manager And Scientists Stand Ready To Provide Personalized Assistance Throughout This Process.

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Recombinant Protein Development

Gene Synthesis

Pilot expression optimization and purification

QC by SDS-PAGE

Monoclonal Antibody Development

Phase I: Antigen Immunization

5 BALB/c mice

1 Pre-immunization sera collection

5 Immunizations

Serum collection

ELISA testing of serum

Monoclonal Antibody Development

Phase II: Fusion & Screening

Harvest Splenocytes For Hybridoma Fusion & Screen For Positive Clones

Selection Of Positive Clones

Monoclonal Antibody Development

Phase III: Subcloning

Subclone & isolate stable positive clones

Final clone isolyping

Monoclonal Antibody Development

Phase IV: MAB Production & Purification

Large scale production of mAb

Purification

QC

Prior to commencing any monoclonal antibody development project, thorough discussions are essential to ensure that crucial parameters are addressed effectively during production. Our specialists are receptive to all customer requirements, which may vary significantly from our standard procedures. Our ongoing objective is to maintain maximum flexibility and ensure the highest possible success rate for your products.

Antibody Conjugation Service

GNG is proud to offer a comprehensive suite of antibody conjugation services, including HRPO (Horseradish Peroxidase) conjugation, biotin conjugation, and gold conjugation. These services are meticulously tailored to meet diverse research and diagnostic needs, providing researchers with versatile tools for a wide range of applications.

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Conjugation to HRPO	Our HRPO conjugation service leverages state-of-the-art techniques to seamlessly attach HRPO enzymes to antibodies, enabling sensitive detection in various immunoassay formats. With precise control over conjugation ratios and optimization protocols, we ensure optimal performance and reliability in every assay.
Conjugation to Biotin	Our biotin conjugation service offers researchers the flexibility to incorporate biotin molecules onto antibodies, facilitating downstream applications such as streptavidin-based detection methods. Through meticulous conjugation processes and quality control measures, we guarantee the production of highly stable and efficient biotinylated antibodies for enhanced assay performance.
Conjugation to colloidal gold	Our gold conjugation service enables the conjugation of antibodies to gold nanoparticles, facilitating applications such as lateral flow assays, immunohistochemistry, and electron microscopy. With expertise in nanoparticle synthesis and conjugation chemistry, we deliver gold-conjugated antibodies with exceptional stability, specificity, and sensitivity.

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