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Phage Display Antibody Library

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Phage Display Antibody Library Services

Discover cutting-edge Phage Display Antibody Library Services with us. Leveraging the power of phage display technology, we provide tailored solutions for discovering high-affinity antibodies to meet your unique requirements. Our high-quality Antibody libraries, enable comprehensive exploration of potential CDR variants, ensuring thorough hit identification.

Our team, equipped with molecular biology expertise and dedicated to innovation, is committed to delivering what you need. Accelerate your monoclonal antibody development program with GeNext Genomics’ phage display services, and receive your antibodies within just 2 months!

Phage Display Technology

Phage display is a selection technique that involves genetic engineering of bacteriophages and repeated antigen-based selection and propagation. It offers a promising alternative for antibody generation outside the immune system, eliminating the need for laboratory animals. Antibody libraries are crafted from genomic information encoding antibody variable domains, sourced from B cells of either native or immune donors.

Antibody library flow
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Diverse population gene pool across various disease background and healthy donors

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DCloning of VH and VL in phagemid vector

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Library preparation and transfection

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Packaging and phage preparation

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Biopanning against the target 4 round

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ScFv generation and sequencing of binders

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Final IgC cloned and expressed in HEK

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Cancer/Infectious proteins injected into mice for immune response

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Cloning of VH and VL and library generation

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Biopanning against target/disease and functional assay

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Identification of ScFv and its genetic sequence

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Final igG cloned and expressed in HEK/CHO

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Application and validation

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Scale up of Antibodies

Our phage display service offerings:
Steps Description Deliverables
Library screening and biopanning Selection and screening of the naïve or immune library. 4-5 rounds of biopanning to enrich binders Progress report
ELISA screening Screening and validation by ELISA to identify binders with desired specificity and affinity Progress report
DNA extraction and sequencing Extraction and sequencing of DNA from identified binders Report DNA sequences
Development of full IgG Gene synthesis Cloning of gene into a proprietary vector Transfection of host cell, screening, scale up & purification Purified IgG antibody
Affinity Maturation Enhancing the affinity and specificity of your antibodies through our affinity maturation services, optimizing their performance for various applications. High affinity antibody
Antibody Characterization Epitope mapping to determine the specific antigen binding site Binding kinetics analysis to measure the strength of antibody-antigen binding Characterization report
Why Choose Us:

Expertise

Our team consists of experts with extensive experience in phage display technology and antibody discovery.

Quality

We prioritize quality at every step of the process to deliver reliable and high-performance antibody candidates.

Customization

Tailored solutions designed to address your specific project requirements.

Timely Delivery

Efficient project management ensures timely delivery of results to meet your deadlines.

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GeNext Genomics

Ready to accelerate your research? Contact us today to
discuss your project requirements and receive a
customized quote.

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